Archive For April 3, 2011

Exome sequencing workflow

By |

This is a high level overview of the general workflow for processing and analyzing whole exome data [thanks Kevin]. QSEQ files are raw reads off the Illumina sequencer. If reads are paired, there there are two files, otherwise there is one file. A Phread score of ~40 is the max (high quality) Demultiplex samples: if…

Read more »