Archive For The “Pacbio” Category

Working with Pacbio IsoSeq data

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Working with Pacbio IsoSeq data

Overview With its long single molecule reads, Pacbio IsoSeq offers the tantalizing possibility of reading entire full-length RNAs. The library prep is not for the faint of heart and a recent provider asked me for 5ug of RNA to start – not an easy task! Moreover, a thorough interrogation of a transcriptome would require at…

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